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Cells were grown in 5 mL of cooked meat broth medium until OD600 reached ~1.
The plasmid was transformed into Escherichia coli BL-21 (DE3) and grown in LB broth medium.
L. lactis NZPyk were grown at 30 °C in GM17 broth medium with or without nisin induction, respectively.
L. bulgaricus CAUH1 was cultivated in de Man-Rogosa-Sharpe (MRS) broth medium and incubated statically at 37 °C.
Protein expression was conducted in E-coli strain BL21 (New England BioLabs, Ipswich, MA) in terrific broth medium.
Degradation by Aspergillus niger was tested on cast films in the presence of Sabouraud's dextrose broth medium.
In vitro anti-tubercular activity was evaluated against Mycobacterium tuberculosis (H37Rv) in Middle brook 7H-9 broth medium.
All cultures were prepared in a selective broth medium for 18 to 24 hours before plating onto sheep blood agar.
E. coli KC6 ΔsmpBΔssrA carrying the plasmid encoding for the respective RNC construct were grown at 37 °C in Lysogeny broth medium to an OD600 of 0.5.
Recombinant constructs were transformed into E. coli BL21 cells (Novagen) and selected clones cultured in the Terrific Broth medium with ampicillin (100 µg/ml).
A single colony of strain ATCC1803 from an anaerobic cooked meat broth agar plate was taken and cultured in 5 mL of cooked meat broth medium overnight.
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