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Limonene was extracted into the hexadecane phase, and negligible amounts of limonene were detected in the cell mass and culture broth (data not shown).
In no case vitamin C was detectable in the culture broth (data not shown).
All mutants displayed a similar growth rate as the wild type ST556 in THY broth (data not shown).
Consistent with the FACS experiments, there was no resolution of the construct in LB broth (data not shown).
Furthermore, smooth-LPS profiles were identical both with and without a final subculture in broth (data not shown), indicating that the strains did not lose the production of the O-antigen chain during the repeated passages on agar medium in this study, as can sometimes occur [22], [24].
Wild-type strains with the ΔtviABCDE10 deletion showed an increase in type 3 secretion when grown in LB broth (data not shown) and all three of the final vaccine strains were resistant to Vi-specific phage and did not agglutinate in presence of anti-Vi antiserum.
Similar(49)
E. ictaluri J100 wild-type, used as control, grew in all fish broth conditions (data not shown).
In the present study, preliminary evaluation revealed that the mycelia crude water extract actively inhibited ACE activity with the IC50 value of 1.134 ± 0.036 mg/mL compared to broth extract (data not shown).
Results showed that cDNA mixes produced the same levels of amplification as those of cDNA from the broth grown bacteria (data not shown), proving that the contents of the pig intestine contained no detectable inhibitory effect on cDNA synthesis and PCR reactions.
The authors pointed out that they also found that increased mortality rate was associated with increased broth microdilution MIC (data not shown), although the trend was not so prominent (2 ).
Both F. novicida strain U112 and the hfq mutant showed similar crystal violet staining when grown in TS broth supplemented with cysteine (data not shown).
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CEO of Professional Science Editing for Scientists @ prosciediting.com