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Broth cultures were grown overnight with shaking.
Broth cultures were grown in a mixture of 50% Brain Heart Infusion Broth, 50% Trypticase Soy Broth and 5 gram/L Yeast Extract supplemented with 0.01 gm/L Sodium Bisulfite, 5 mg/L hemin and 5ug/L Vitamin K.
Broth cultures were grown to optical densities (OD600) between 0.50 and 1.00.
These broth cultures were grown at 37°C on a rotator for 2 hours and were moderately turbid.
MSSA and MRSA broth cultures were grown in MHB and MHB + 2% NaCl, respectively, until an exponential stage (2 × 10 CFU mL−1) was reached (see Section 2.3).
These broth cultures were grown at 37°C on a rotator for either 2 hours, in the case of Hib 10810, or for 3 hours, in the case of R2866, and were moderately turbid.
Similar(54)
Luria broth culture was grown as 60 mL of culture in a 100 mL flask.
P. aeruginosa strains (Table 1) were routinely propagated at 37°C on Luria-Bertani (LB) agar or LB broth [ 40] and all liquid cultures were grown with shaking (225 rpm).
To study the effect of various culture media on the expression of HIV-1 CA, cultures were grown in 1% glucose-supplemented M9 minimal broth, LB Broth, Terrific Broth, and Super Broth essentially following the expression protocol given above.
The colonies were picked up and the cultures were grown in 7H9 broth.
For all strains, cultures were grown in Nutrient Broth (NB; Merck) medium (28°C, 180 rpm).
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