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Bring an Asian broth base in from home: in a little jam jar add a dash of soy sauce, fish sauce, and a little grated ginger and garlic.
All solvents were purchased from Carlo Erba (France), Middlebrook 7H9 TB broth base was obtained from HIMEDIA (India), Glycerol (AR) obtained from Lab Equip Ltd (Tanzania), iodonitrotetrazolium (INT) chloride, Isoniazid (R&D) were purchased from Sigma (UK).
The most important medium remnants in BFC-1 are soy hydrolysate and yeast extract, inherent components of the Select APS LB Broth Base used for bacterial growth and bacteriophage production.
The presence of Campylobacter was determined by incubating the remaining enrichment broth microaerobically (2% H2, 5% O2, 5% CO2, balance N2) 100-ml volumes of nutrient broth base (Mast, Bootle, UK) with 5% horse blood, growth supplement (Mast Selectavial SV61), amphotericin (2 µg/ml), cefoperazone (15 µg/ml) and trimethoprim (10 µg/ml) at 37°C.
PPLO broth base (Difco), 20% (vol./vol)./vol
Escherichia coli strain S17-1 λpir/pSAM_Bt was grown at 37°C under aerobic conditions in Luria broth base (LB) and Luria agar (BD Biosciences).
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Type II models were defined as models computing productivity as the sum of local productivities within the cultivation broth (based on the light intensity locally experienced by individual cells) without consideration of short light cycles.
The icaA/icaD genes were found in 32 S. aureus and 5 other strains and these results was perfectly correlated with results on Congo-red broth based on TSB supplemented with sucrose.
The method included an 18 h non-selective enrichment in buffered peptone water (BPW) and a 6 h selective enrichment in Rappaport Vasilliadis Soya (RVS) broth, based on the traditional culture method, ISO 6579 2002.
Mussels marinière, steamed in white wine and garlic, anchors the list, but the variations spin off from there, with mussels in a broth based on Billy Bi soup, a blend of vermouth and saffron cream, and Portuguese-style mussels in a broth flavored with chorizo and cilantro.
So a good strategy when you hit the salad bar is to start off with a broth based soup.
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