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Brain and hypophysis from broodstock animals were also dissected and rapidly flash frozen in liquid nitrogen.
For 330 out of a total of 392 genotyped broodstock animals, both alleles at the underlying polymorphism could be deduced.
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None of the broodstock was affected, even though these animals were in close spatial association with the affected tanks.
The parents of the mapping population can, in the context of the present study, be regarded as random animals sampled from the broodstock population.
A broodstock design that encompasses sufficient numbers of animals that reflect the genetic, and preferably ecological, identity of the stocks to be enhanced should be adopted.
An additional 214 animals from among the 2005 broodstock (i.e. the parents of the 2005 generation; Figure 4) were therefore genotyped for the four-marker haplotype.
About 476,000 fish have been released -- including 3,000 surplus broodstock 16 to 26 inches long.
"They are the originals," said Corene Jones, 39, the broodstock coordinator for the Lahontan National Fish Hatchery in Gardnerville, Nev.
A reduction in allelic richness (Ar) was identified from broodstock to offspring at 1 dph, (Ar was 3.94 among broodstock and 3.52 among offspring sampled).
The major factors influencing design plans are the number and size of broodstock on hand and the anticipated spawning rate of those broodstock.
The broodstock have been separated into different tanks, each with the light set to the photoperiod that would be typical of each of the year's months.
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