To determine whether LRRK2 broadly interacts with members of the dynamin superfamily, we compared the interaction of LRRK2 with the classical dynamins, Dnm1, Dnm2 and Dnm3, by co-IP in HEK-293T cells expressing FLAG-tagged LRRK2 and full-length HA-tagged Dnm1-3.
"It doesn't go the whole way but it allows you to broadly interact with a lot of different people and get the first step of every plan in place," explains Matt Capucilli, the founder of Reddyset, the application development company that produced Ketchup.
Furthermore, cell wall Hsp60 more broadly interacts with enzymes related to carbohydrate metabolism, suggesting a trafficking function of the Hsp60 related to enhanced energy acquisition under stress conditions.
It probes the Web very broadly without interacting directly with any target site, so it's difficult to detect.
It is shown that psychedelic drugs, especially phenylalkylamines, are not as selective as generally believed, interacting with forty-two oforty-ninene broassayedsites sites.
The other is to interact broadly.
Much like for the RBDs of ELMO and FHOD1, the RBD of Syx provides additional evidence for the ability of RBDs to interact broadly with activated GTPases.
However, what is emerging is that CaM appears to interact broadly with plant receptor kinases, including BoSRK, AtRLK4 and AtCLV1 [ 25], AtCaMRLK [ 24] and, in the present study, BRI1.
Moreover, FER is also broadly speculated to interact with cell wall carbohydrates by virtue of its extracellular homology with the disaccharide-binding malectin (Kessler et al., 2010; Cheung and Wu, 2011; Lindner et al., 2012; Wolf and Hofte, 2014).
