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The latter, although less disruptive to the protein's tertiary structure than the former, would be expected to result in great local flexibility, a prediction that is consistent with the notably broader temperature transition for protein unfolding compared to cpOYE291 and -292.
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The aqueous solution of P NOEG- r-NCA 4:1 r-NCA 4s a phasexhibitstion over a broader temphaseure range (60–80 °C) atransitiontratioverf 1.5 g/L and broader no longer temperaturensive when investigated at a concentrangen of 1.0 g/L and beyond.
The expected "high temperature transition" (HTT) was observed (between 35 °C and 55 °C), but this was preceded by a broad "low temperature transition" (LTT) event occurring over a range of up to 20 °C.
Other studies involving heat shock employed a broader temperature change.
The temperature transition rate was 20°C/second.
The temperature transition rate was 0.1°C s−1.
The temperature transition rate was set at 20°C s−1.
The temperature transition rate was set at 20°C per second.
The temperature transition rate was set at 20°C/ sec.
The temperature transition rate was 20°C/second for all steps.
The temperature transition rate was in each cycle 20°C/s.
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