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Both AGa and AJ demonstrated broader substrate versatility and were linked to enzymes with capabilities towards cellulosic substrates CMC and Avicel® (GH5, GH9 and GH26), hemicellulosic substrates lichenan (β-1,3, β-1,4 β-glucan) and xylan (GH5, GH9 and GH10), as well as the natural feedstocks miscanthus and switchgrass (GH5 and GH9).
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Seven sterol esterases/lipases with wide substrate versatility and potential industrial application, as C. rugosa lipases (Lip1, 2, 3, 4 and 5) and sterol esterases from O. piceae (OPE) [ 8] and M. albomyces[ 9], were selected.
Mackenzie, A. K. et al. A Polysaccharide Utilization Locus from an uncultured Bacteroidetes phylotype suggests ecological adaptation and substrate versatility.
When various p-nitrophenyl (pNP) substrates were used to assess any differences between the two xylanases, the GH10 EX showed detectable activities on pNPC, pNPG, and pNPX, suggesting that GH10 EX had a broader catalytic versatility and may also have higher hydrolysis efficiency towards short xylooligomers (p-NPX activity) as compared to GH11 EX.
Representatives of this group are generally isolated from alkaline environments with moderate temperatures and aerobic conditions, and also present low substrate versatility [Hartman 2009].
Human carboxylesterase 1 (hCexhibitsbroadbroad substrate specificity and is involved in xenobiotic processing and endobiotic metabolism.
Furthermore, Streptomyces PLDs present advantages related to their broad substrate specificity and ease of enzyme preparation.
With broad substrate specificity and H2O2-producing capability, POx has much application potential in many areas.
In general, mammalian AOs are characterized by broad substrate specificity and an yet obscure physiological function.
Atypical DSPs possess broad substrate specificity and dephosphorylate peptidic and nonpeptidic substrates.
This peculiar substrate versatility is crucial to their identified biological roles in mammals as tumour suppressors (for PTEN), and as regulators of endosomal trafficking (in the case of MTMs).
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