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Unlike classical eukaryotic PPP, which are specific for Ser and Thr residues, bacterial PPPs may have broader substrate specificity, and the cold-active phosphatase originally described in Shewanella is a Tyr PP (Tsuruta et al., 2008).
Vaccinia virus thymidylate kinase, although similar in sequence to human TMP kinase, has broader substrate specificity and phosphorylates (E -5- 2-bromovinyl -dUMP and dGMP.
Therefore efficient screening techniques for TAs with high activities as well as broader substrate specificity and different enantioselectivities are crucial for the successful application of transaminases for the synthesis of β-amino acids.
(1081) PEPT1 has a broader substrate specificity and was examined as a means to enhance intestinal absorption of ester prodrugs.
The repertoire of A3 proteins is additionally expanded through splice alternatives and read-through mechanisms, resulting in broader substrate specificity and finer regulation of DNA modification potential.
This is consistent with previous reports that established MSL proteins as the main enhancers of MOF's H4K16 acetylation activity, while the NSL complex was shown to possess broader substrate specificity and can crosstalk with histone methylases (Cai et al., 2010; Kadlec et al., 2011; Zhao et al., 2013b).
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This approach allowed us to successfully identify GH1 enzymes with sought-after specifications, notably activity at high temperatures in the presence of IL, broad substrate specificity, and activity against more complex substrates such as laminarin, that are potentially useful in a biotechnological setting.
With broad substrate specificity and H2O2-producing capability, POx has much application potential in many areas.
Furthermore, Streptomyces PLDs present advantages related to their broad substrate specificity and ease of enzyme preparation.
Human carboxylesterase 1 (hCexhibitsbroadbroad substrate specificity and is involved in xenobiotic processing and endobiotic metabolism.
GDSL esterases and lipases are hydrolytic enzymes with multifunctional properties such as broad substrate specificity and regiospecificity.
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