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The two tissues used for the deep RNA-seq of L. menadoensis were both richer than muscle, although testis resulted to be, by far, the one expressing a broader range of transcripts.
Although this sampling process has been highly effective, a determination of the exact composition of the pea transcriptome will require a corresponding genomic sequence assembly, permitting annotation and classification of a broader range of transcripts.
Because RNA-Seq detects a broader range of transcripts than microarrays, which are limited to the probe set, any genes not found on the array were first filtered from the RNA-Seq data.
Highly specialized tissues are expected to invest the most gene expression in a selected set of genes, thus being transcriptionally poor, whereas tissues involved in many different biological functions, characterized by the coexistence of many different cell types are expected to be transcriptionally rich, as they express a broader range of transcripts.
Testis expresses a broader range of transcripts, which is in agreement with the assumption that cells in this organ are characterized by drastic morphological and functional changes linked to gamete generation: in this scenario chromatin structure is constantly rearranged and gene expression may therefore be substantially variable during the different stages of spermatogenesis [ 66].
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This criterion should be evaluated for a broad range of transcripts.
To explore the broadest range of transcripts, Affymetrix extended probes were imported, yielding transcripts of which 112,207 have annotation with 50,601 at the gene or mRNA-level.
The length wise distribution of the unigenes is presented in Additional file 3A, indicating the trancriptome with broad range of transcripts.
This in turn provides a broad range of transcripts and pathways that could potentially be modulated by small RNAs, perhaps including siRNAs that function as microRNAs.
A broad range of transcripts indentified in this study were associated with amino acid metabolism and biosynthesis; based on the KEGG databases [ 36], 18 of the 22 amino acid biosynthesis pathways were represented by 100 454 contigs.
For sequencing the whole transcriptome we used RNA samples from nine different conditions (exponential growth phase, heat and cold shock, salt stress, oxidative stresses, and ethanol stress) to obtain a broad range of transcripts.
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