Broad localization of PEAR1 GFP is detected at the level of vascular initial cells.
It may be that the broad localization of N-Mis17 in the nuclear chromatin directly or indirectly downregulates Mis6 complex that is bound to the centromere (depicted in Figure 6B).
d, The expression of six PEAR genes (including PEAR1, PEAR2, DOF6, TMO6, OBP2 and HCA2) shows a similar pattern of expression to that of PEAR1, in which both mRNA and transcriptional fusion reporter exhibit a PSE-specific pattern with a broad protein localization.
A polyclonal antibody against UNC-34 shows broad cortical localization, as well as enrichment near apical junctions in epithelial cells.
These proteins, eIF4A, Eno2p, and Pdc1p, have a broad cytoplasmic localization (Campbell et al., 2005; Huh et al., 2003).
The broad intracellular localization of OCRL may reflect its importance in preventing the build-up of ectopic intracellular pools of PI 4,5 P2 and PI 3,4,5 P3.
The results, illustrated in Figure S5B, show that all three factors have a broad nuclear localization, with neither enrichment nor exclusion underlying Xist RNA domains, as assessed by co-staining for H3K27me3.
Nevertheless, considering the position of events captured by genotype arrays reveals broad-scale localization differences, with male events more prevalent in the telomeres and female events relatively dispersed throughout the genome.
Secondly, at a broader level, the localization in open chromatin, and consequently low noise, genomic regions could also contribute to the small level of noise detected [ 15].
Although TMO6 mRNA is highly specific to PSE cells, its transcriptional fusion reporter shows a broad but PSE-abundant expression pattern, with a broad TMO6 protein localization.
