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As previously established in other studies, the absolute magnitudes of normalized signals reflects the broader dynamic range of the qPCR method compared to microarray measurements [ 53, 64].
However, other neurons within or outside the cortical column (e.g. in the colliculus; Cohen & Castro-Alamancos, 2010) may show a broader dynamic range of firing output at larger stimulus intensities.
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However, detection of small nutrients is challenging, considering the high diversity and broad dynamic range of molecules in food samples.
There are many challenges for the study of proteins in biological systems, including a broad dynamic range of expression, heterogeneity, complexity of modifications and many technical and preparatory steps prone to the introduction of bias.
Small nutrients detection is challenging due to the high diversity and broad dynamic range of molecules in food samples, and a further issue is to track low abundance toxins.
Here, we demonstrate computationally how two classic genetic oscillators, the dual-feedback oscillator and the repressilator, can be re-designed to provide independent control of amplitude and period and improve tunability that is, a broad dynamic range of periods and amplitudes accessible through the input "dials".
A broad dynamic range of standards was used to account for the varied expression levels of different targeted genes.
The generic model exhibited the dramatic amplification and broad dynamic range of gradient-sensing in wild-type cells, as well as defective polarization for "mutant" parameter values.
This balance of cooperativity and positive feedback was able to reproduce both the broad dynamic range of wild-type cells and the reduced dynamic range of the mutant cells.
The broad dynamic range of mRNA-seq analysis makes it possible to detect transcripts that are present at very low abundance, easily below the range of 1 tag/M.
Importantly, the method presented here has been thoroughly characterized and shows a broad dynamic range of accurate [D4] 4 quantification, making it applicable in future studies.
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