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The decoupling of the conformational broadening dynamics between different regions of the protein induced by the H87V mutation clearly indicates that the apparent global character of the motions in this timeframe proposed in previous relaxation studies [ 13, 14, 16] is coincidental.
Our NMR relaxation studies of FKBP12 variants demonstrate that the conformational broadening dynamics described previously appear to involve at least three distinct processes centred on the 80′s loop, the 50′s loop + α-helix and the 40′s loop respectively.
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This structurally conservative mutation also serves to suppress the much more rapid conformational line-broadening dynamics in the 80′s loop and partially suppress mobility in this time regime in the region surrounding the start of the α-helix.
It also allows the incorporation of seed banks and other dormant life forms, thus broadening patch occupancy dynamics to include sink habitats.
Introduction of the P119L mutation into FKBP52 induces line-broadening dynamics in this loop, although comparison of the 2D H,N-HSQC spectra indicated minimal change in structure (Supplementary Figure S4 at http://www.biochemj.org/bj/461/bj4610115add.htm).htm
When ADP is bound, the enzyme's resonances undergo significant line broadening originating from conformational dynamics on the microsecond to millisecond time scale similar to those experienced with ATPγN or ATP.
Improvements of this description, on the basis of approaches developed in computational fluid dynamics are discussed, broadening the outlook given in Bufferand et al.
Although FKBP12 also exhibits line-broadening conformational dynamics in the β4 β5 loop, that transition appears to be centred around the flip from a positive to a negative main-chain ϕ torsion angle at Gly [ 58].
These vast mobile data offer new perspectives and approaches to measure economic dynamics, and they are broadening the social science and economics fields.
We employed [P18A]-Trp-cages for our remaining studies, with the exchange line broadening at Ala18-Hβ as the dynamics probe.
Understanding disease dynamics and tracking outbreaks requires broadening consideration to the community level instead of solely focusing on individual host-pathogen interactions.
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