In an effort to broaden the obtained gene expression profile in G. sinensis, total RNA was extracted from four different tissues (tender shoots, young leaves, flower buds, and round thorns) and mixed in equal parts for sequencing using the Illumina platform.
To broaden the WRKY gene family expression profile obtained with the OsWRKYARRAY, WRKY expression data from the 22 K NIAS array (National Institute of Agrobiological Sciences) were extracted to highlight those genes that are co-expressed in a wider range of abiotic stress conditions, as well as at different developmental stages (shoot, meristem, panicle).
Our approach to dealing with causal direction is to broaden the context beyond mRNA expression and extract information from high-throughput data about auxiliary variables associated with each gene.
On the parody site, which mimics the design of a legitimate M.I.T. Web page, the Erotic Computation Group (ecg.media.mit.edu) claims to study "the implications of modern technology on human eroticism in its myriad forms" as well as ways to "broaden the range of human amative expression".
In order to corroborate these data and to increase the relevance of the results, we decided to broaden the study and analyzed TLR3 expression in a panel of six human breast cancer cell lines after 9cRA treatment.
In order to validate and extend these results, we broaden the analysis and studied TGFBI expression in a large series of samples obtained from stage I IV NSCLC patients.
By establishing which epigenetic configurations are involved in governing monoallelic gene expression, we will broaden the understanding of epigenetic mechanisms as they relate to cancer progression and cellular differentiation.
We anticipate regular expansions of the database to broaden the range of developmental and temporal expression profiles included.
To broaden the application of this strategy, it is highly desirable to reduce the overall size of the expression cassettes.
