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We performed a diagnostic study by first developing a clinical prediction model, with derivation and cross validation in two Dutch populations (Erasmus MC-Sophia and Haga-Juliana children's hospitals, the derivation population), then externally validating the prediction model in a UK population (Coventry, broad validation population).
Secondly, we derived and cross validated a prediction model using two large prospective cohorts and we assessed broad validation in an external population from a different country.
To our knowledge, only limited information is available about false positive/negative measurements after Transplex amplification relative to standard procedures for expression profiling [10], [24]; moreover, broad validation by qPCR has not been performed.
Currently available alternative methods for expression profiling from nanograms of RNA or from very small cell populations lack a broad validation of results to provide accurate information about the measured transcripts.
for broad validation of similarity between replicates.
Broad validation of PBPK models describing partitioning of compounds from sediment and water into fish.
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Through the broad validations in China's mega-cities, this system has demonstrated capabilities of simplifying, appropriately quantifying, and evaluating the complex process of urban planning and management towards sustainability.
Obviously, further work in broader validation of this conclusion is needed using other data sets as well as considering other comparison criteria.
Obviously, further work in broader validation of this conclusion is needed using other data sets as well as other comparison criteria.
Here, we present the broadest validation of a rigorous free energy-based approach applied to protein stability reported to date: 700 + single-point mutations spanning 10 different protein targets.
A broader validation of the results of this research using data from cities across the U.S. with different weather systems is warranted.
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