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However, a broad temperature gradient of 80 K developed inside the reactor.
There is normally a broad temperature gradient behind the boundary with more widely spaced isotherm packing.
TGCE analysis was performed in a 96-capillary array instrument (SpectruMedix SCE9612), in which PCR-amplified DNA and cDNA samples were separated by capillary electrophoresis in a single broad temperature gradient.
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The combined broad-range PCR-TTGE (temporal temperature gradient gel electrophoresis) technique can detect several bacterial pathogens in ticks in a single step.
In a broad temperature range, the pure valley current is found to be linearly dependent on the temperature gradient while it increases with the increasing temperature of one lead for a fixed thermal gradient.
The optimal annealing temperature of each mismatched oligonucleotide combination was determined by measuring the rate of dsDNA disassociation relative to the observed Tm, i.e., the slope of the gradient formed between Tm ± 1°C in the disassociation profile, across a broad temperature range of 20°C to 80°C in 5°C increments.
This allows measurement of the temperature gradient.
The temperature gradient in the furnace was fixed at 0.6 °C/mm.
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) for studying soil microbial communities.
strongest effect on the north-south temperature gradient.
§ 3252.15 When must I abandon a temperature gradient well?
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