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We conclude that SATé is highly efficient at producing accurate multiple sequence alignments for large numbers of divergent ITS sequences from a broad taxon sampling in ascomycetes.
This underscores not only the importance of broad taxon sampling, but also the instability of †Mesonychia and †Indohyus, particularly when compared to the relatively stable phylogenetic 'backbone' of Artiodactyla that is supported by data from extant lineages.
Thus advance of lepidopteran mitogenomics deeply requires new genomes derived from a broad taxon sampling.
For the first time a broad taxon sampling of all major euthyneuran subgroups is considered.
However, whether the cox1 barcode sequence could be applied to the whole Orthoptera requires broad taxon sampling.
With its broad taxon sampling, this study is the hitherto most comprehensive analysis of metazoan CS evolution.
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In the THERMORANK analysis, for example, A. pompejana ranks behind H. robusta and L. gigantea, also highlighting the importance of broad taxon-sampling when performing these comparisons.
Broad taxon-focus and species-specific analytical studies of 'dynamic biogeography' have demonstrated that many species persist in peripheral subpopulations rather than core areas of their geographical range [ 11, 12], although extinction rates may also be higher at range edges [ 13, 14], making it difficult to predict the likelihood of brown bear survival in the peripheral Irish refugium.
However, broader taxon sampling of group II introns is needed to reliably infer their evolutionary history.
Broader taxon sampling is crucial not merely because it may help break up long branches.
However this observation needs to be verified further using broader taxon sampling.
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