Both the lack of oxygen reactivity and the broad substrate tolerance make PDH attractive for the fabrication of amperometric biosensors and small self-powering devices: enzymatic biofuel cells (EBFCs).
Ease of handling, broad substrate tolerance, high stability towards temperatures and solvents, high enantioselectivity, convenient commercial availability, and reusability are the key advantages of choosing lipase as a biocatalyst in a huge number of organic transformations.
The enzymatic oxidation of non-activated aliphatic prim-alcohols by alcohol oxidases shows a remarkably broad substrate tolerance (Table 1) and encompasses straight-chain or branched substrates with chain lengths ranging from C1 to C16.
The Mitsunobu reaction is renowned for its mild reaction conditions and broad substrate tolerance, but has limited utility in process chemistry and industrial applications due to poor atom economy and the generation of stoichiometric phosphine oxide and hydrazine by-products that complicate purification.
After the shift to a new host plant only the selective transport element needs to adjust to the new metabolites; all other transport elements may remain unchanged due to their broad substrate tolerance.
A series of novel pyridine stabilized Pd NHC complexes were developed, which revealed high activities and broad substrates tolerance in the amination of various (hetero -aryl chetero -aryl
Self-resistance might, therefore, stem from the efficient export of abyssomicin from the cell; the low level of abyssomicin production observed in the Δ abyC and Δ abyD strains can be explained by a degree of passive diffusion from the cell or by partial suppression of Δ abyD by another MFS transporter with broader substrate tolerance.
By manipulating the order of addition and identity of enzymes and exploiting their broad-substrate tolerance, we engineered the production of highly unnatural derivatives, including an N-C peptide macrocycle of 22 amino acids in length.
The high substrate tolerance of these enzymes towards different FA and alcohol moieties resulted in the biosynthesis of a broad range of MBE.
