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In vitro processing of semisynthetic substrates unrelated to the prelacticin 481 peptide demonstrated the broad substrate tolerance of LctM.
Ease of handling, broad substrate tolerance, high stability towards temperatures and solvents, high enantioselectivity, convenient commercial availability, and reusability are the key advantages of choosing lipase as a biocatalyst in a huge number of organic transformations.
By necessity, an enzyme that is capable of modifying 29 diverse precursor peptides must exhibit broad substrate tolerance.
Both the lack of oxygen reactivity and the broad substrate tolerance make PDH attractive for the fabrication of amperometric biosensors and small self-powering devices: enzymatic biofuel cells (EBFCs).
After the shift to a new host plant only the selective transport element needs to adjust to the new metabolites; all other transport elements may remain unchanged due to their broad substrate tolerance.
The enzymatic oxidation of non-activated aliphatic prim-alcohols by alcohol oxidases shows a remarkably broad substrate tolerance (Table 1) and encompasses straight-chain or branched substrates with chain lengths ranging from C1 to C16.
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A series of novel pyridine stabilized Pd NHC complexes were developed, which revealed high activities and broad substrates tolerance in the amination of various (hetero -aryl chetero -aryl
Self-resistance might, therefore, stem from the efficient export of abyssomicin from the cell; the low level of abyssomicin production observed in the Δ abyC and Δ abyD strains can be explained by a degree of passive diffusion from the cell or by partial suppression of Δ abyD by another MFS transporter with broader substrate tolerance.
By manipulating the order of addition and identity of enzymes and exploiting their broad-substrate tolerance, we engineered the production of highly unnatural derivatives, including an N-C peptide macrocycle of 22 amino acids in length.
Plasmodium falciparum thymidylate kinase (PfTMPK) shows a broad range of substrate tolerance when compared to the corresponding human enzyme.
They have received increasing attention due to their broad substrate specificities and tolerances against high temperatures and organic solvents (Li et al. 2013; Zhou et al. 2013), which are favorable characteristics of biocatalysts for chiral alcohol synthesis (Alsafadi and Paradisi 2013).
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