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The homodimeric enzyme has a broad substrate spectrum and an excellent stereoselectivity, but is rapidly inactivated at aqueous organic interfaces.
As a soluble prokaryotic P450 with broad substrate spectrum and hydroxylating capacity, CYP106A2 is an outstanding candidate to establish bioconversion processes.
The recombinant AcCR exhibited excellent enantioselectivity, broad substrate spectrum, and highly stereoselective anti-Prelog reduction of prochiral ketones.
MamK from Mcas exhibited a broad substrate spectrum and could hydrolyze various nucleotides (ATP, GTP, ADP and GDP) and release Pi (Fig. 2A).
As shown in Table 4, AcCR exhibits a broad substrate spectrum and the enantioselectivity of this enzyme follows anti-Prelog rule which are relatively rare in nature (Tang et al. 2014; Li et al. 2015).
These enzymes have a broad substrate spectrum and might form a starting point for the evolution of enzymes for regio-specific prenylation of aromatic small molecules.
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A key step in fulfilling this potential is the discovery of new TAs with a broader substrate spectrum and different enantioselectivity.
The broad substrate spectrum of HRP and the spontaneous polymerization of radicalized reaction products in particular facilitate the use of HRP in detoxification applications.
Recently, we isolated a kefir yeast, Kluyveromyces marxianus KY3 (data not shown), that has the potential to serve as a host for bioethanol production and a biorefinery platform, because the strain has broad substrate spectrum, including both hexose and pentose sugars, and produce valuable flavor byproducts such as 2-phenylethanol.
Intriguingly, TACE possesses a broad substrate spectrum of growth factor and cytokine substrates, whose cleavage leads to activation of the epidermal growth factor receptor (EGFR) signalling cascade (e.g., TGF- α and amphiregulin) (Yarden, 2001; Sunnarborg et al, 2002; Wang et al, 2009).
We concluded that CotA from B. pumilus has great potential as biocatalyst due to its thermo stability, pH optimum in the neutral to alkaline range and broad substrate spectrum.
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