Three Streptomyces genes, NphB, SCO7190, and NovQ, whose gene products have broad substrate specificity, were overexpressed in a model legume, Lotus japonicus, in the cytosol, plastids or mitochondria with modification to induce the protein localization.
It is suggested that phytases with broad substrate specificity are better suited for animal nutrition purposes than phytases with narrow substrate specificity [39].
(1203) The pregnane X receptor, exhibiting a broad substrate specificity, is involved in transcriptional regulation of PGP and multiple CYPs.
Nevertheless why mammals have three ABCD proteins with distinct but overlapping specificity whereas most plants and yeast have a single one with apparently broad substrate specificity is unclear.
Similarly, using binding sensitivity to proteases such as trypsin and chymotrypsin, which have broad substrate specificities, is only rarely helpful because a large number of receptors are sensitive to these enzymes.
However, broader substrate specificity is probably more common for transporters.
This observation conforms to the trend that sugar-specific kinases are typical in prokaryotes and unicellular eukaryotes, whereas hexokinases with broader substrate specificities are typical in multicellular eukaryotes [89].
While, some GTs with broad spectrum of substrate specificity are promising candidates for glycoengineering while others with stringent specificity pose limitations in accepting molecules and performing catalysis.
Cytochrome P450s (P450s) are the most versatile biological catalysts in plants; however, because the structure of the P450s has not been fully established, their broad substrate specificity has been limitedly discussed.
Secondly, although it has been reported that MSH2 has broad substrate specificity, there is a relative lack of information regarding the effects of MSH2 deficiency in cells treated with differing forms of oxidative stress.
