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After overexpression in Escherichia coli, the purified protein was shown to have a broad substrate range including adipate.
In contrast to MPH, OPAA has a relatively broad substrate range, including diisopropyl fluorophosphate (DFP), cyclohexyl methyl fluorophosphate, paraoxon and the G-series nerve gasses (sarin, soman and tabun), which are turned over more rapidly than the 'model' substrate methyl parathion (kcat of 583 s−1 for DFP; Defrank and Cheng 1991).
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WS from A. baylyi ADP1 exhibited a broad substrate range that included linear alcohols from C2 to C18 (Table 1).
The distinct metabolic and physiological characteristics of thermophiles, including broad substrate range and high uptake rates, coupled with recent advances in genetic tool development, present unique opportunities for strain engineering.
The use of cytochrome P450 (P450 or CYP) enzymes as biocatalysts for the production of fine chemicals, including pharmaceuticals, has been of increasing interest, primarily owing to their catalytic diversity and broad substrate range.
Therefore, the equivalent residues different between Ancestor 1 (MphI and MphK) and Ancestor 2 (broad substrate range) may be determinants of substrate specificity46.
Korshunova, Y. O., Eide, D., Clark, W. G., Guerinot, M. L. & Pakrasi, H. B. The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range.
Localization of broad substrate range enzymes and their close proximity for efficient formation of enzyme substrate complex are the significant factors for increasing the efficiency of the cellulosomes.
A study on Pseudomonas putida originally isolated and characterized one FACS with a broad substrate range [34].
DyPs exhibit a very broad substrate range.
WS from M. musculus C57BL/6 also exhibited a broad substrate range (Table 1).
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