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The broad substrate acceptance of GST A1-1 contrasts with the high selectivity of GST A4-4 and GIMFhelix for alkenal substrates.
The recently characterised 5-hydroxymethylfurfural oxidase from Methylovorus sp. MP688 showed a broad substrate acceptance of various furfuryl alcohols (Table 4, entry 42), but it also showed activity on benzylic alcohols with substituents in para-position (Table 4, entries 3 and 5) and vanillyl alcohol (Table 4, entry 15) (Dijkman and Fraaije 2014).
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Conservation usually indicates preservation of a particular function, and in the Mu class, it would appear that the conserved Ile10 is not necessary to maintain catalytic functions but to prevent loss of broad substrate acceptance.
The broad substrate acceptance is consistent with the possibility that multiple paths lead to the same intermediates, and that competition could occur in vivo.
Reetz, M. T., Bocola, M., Carballeira, J. D., Zha, D. & Vogel, A. Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test.
For substrate acceptance of GOase, the axial position of the C4 position is crucial.
The reportedly broad substrate specificity of Oplophorus luciferase also afforded the opportunity to design an improved luminogenic substrate.
Due to their broad range of keto acid substrate acceptance, the leuABCD-operon enzymes are proposed to facilitate a direct carbon chain elongation from the central intermediate pyruvate to the branched chain amino acid precursor 2-ketobutyrate which is synthesized from threonine under standard conditions.
The stromelysins have relatively broad substrate specificity, catalyzing degradation of many different substrates in the ECM [ 1**, 2**].
Therefore, the equivalent residues different between Ancestor 1 (MphI and MphK) and Ancestor 2 (broad substrate range) may be determinants of substrate specificity46.
Except for testing their substrate acceptance, also the enantioselectivity of some selected BVMOs was studied.
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