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However, the very broad solute specificity of Aap and Bra made it difficult to determine which amino acids are responsible for this phenotype.
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This hypothesis may be supported by the broad substrate specificity of KDPG aldolase identified biochemically [28] and structurally [29].
Fad enzymes, including the broad substrate specificity of the FACS, have also been characterized in Pseudomonas fragi [31] [33].
We have also further elucidated the mechanism for the broad substrate specificity of potentiated Hsp104.
Nevertheless, the broad substrate specificity of TrxR1 plays important physiological roles.
This explains the broad substrate specificity of the overall detoxification process [ 21].
This may be done to model the broad substrate specificity of the enzyme or to indicate that the exact substrate specificity is unknown.
However, we had previously identified a region likely to be important in solute specificity in AapQ [12] and mutation of P144D reduced branch chain amino acid transport by ∼60%.
However, the mechanisms that support the broad specificity of DcR3 remain to be fully defined.
The initial inhibitors were pre-existing clinically used compounds and exploited the broad specificity of P-gp.
This may also support the broad specificity of the enzyme.
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