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In this issue, a paper by Rapp et al. [ 3] tackles the question of how broad-scale gene expression can change with domestication by contrasting patterns of gene expression in developing fiber cells from domesticated and wild cotton.
This finding suggests that broad-scale gene expression changes are similar in frogs challenged by Bd regardless of their previous infection history, and only a small subset of genes are differently expressed among naïve and previously infected individuals.
Large-scale gene expression studies frequently employ microarray technologies.
The composition of the EST set used in these microarray experiments has previously been considered specifically with respect to the complement of developmental signalling pathway components [ 2, 3], but this paper is the first to examine broad scale changes in gene expression during development for any cnidarian.
Few methodological approaches offer the means to explore this question on as broad a scale as gene expression profiling.
Extracted mRNAs were used for large-scale gene-expression profiling.
The current expression analysis extends these findings by demonstrating broad coordinate gene expression in immunological pathways.
These questions may be answered after broad-scale application of gene expression assays, such as RNA-seq.
Gene expression analysis indicated that CD99 forced expression induced broad gene expression down-regulation: almost 80%% of genes showed decreased expression in CD99 transfected cells.
At the broad gene expression level, more genes are misexpressed between species compared to hybrids.
There have been few comparisons of broad gene expression profiles in men and women.
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