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Pneumocystis carinii has been shown to synthesize cholesterol, and a broad sampling of species of Taphrina and Protomyces have 24-methyl-cholesta-5,22-dienol (brassicasterol) as their primary sterol.
Figure 1 provides a representation of SBP2/SBP2L protein topology as a global sequence alignment that includes examples of SBP2 and its homologues from a broad sampling of species that are known to express selenoproteins.
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Because carrion flies are notoriously indiscriminate eaters, they yield DNA from a broad sample of species.
This finding, covering a broad sample of species whose vocalizations are fairly diverse, indicates that the intense selection on certain call characteristics observed in many anurans does not eliminate all acoustic indicators of relatedness.
It should be emphasized that as a result of our "thinly sampled" experimental design (relative to land-plant phylogeny as a whole), the maximum resolution that we report here should tend to be on the upper end of what is possible with a broader sampling of species and populations in the clades we considered.
Species chosen for alignment represent all the five subfamilies currently recognized for the Orchidaceae [ 58, 60] with a broader sampling of species from the largest subfamily Epidendroideae.
The heterogeneous results from our analysis demand further research on the transcriptome of dogroses which considers a broader sampling of species and genes and accounts also for possible tissue-specific differences.
Future studies should consequently include a broad sampling of this species over adjacent mountain ranges to explore how such distribution oscillations have affected current levels of genetic diversity in these populations.
Initially, contiguous sequences including ITS1 and ITS2, 5.8S and approximately 600 bases of the 5' terminal domain (D1/D2 regions) of the nucLSU of a broad sampling of Phlegmacium species by Garnica et al. [[ 13, 15], unpublished data] were automatically aligned in MAFFT v5.7 [ 55] and analysed by neighbor joining [ 56] in PAUP* 4.0b10 [ 57] using Kimura 2-parameter distances [ 58].
The broad sampling of arthropod species that revealed the absence of any CYS/CAS-like sequence (even as a distant recognizable trace) argues against the origin of the gene in the common ancestor of tetranychid mites and Lepidoptera, followed by multiple independent losses.
To examine whether lowered selective constraints have resulted in a more extreme amino acid bias in A. kona mtDNA, we examined the amino acid compositional heterogeneity for a set of 19 mitochondrial proteins (mainly involved in OXPHOS pathway) among a broad sampling of taxa (54 species).
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