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Primers (10 µM) were determined to amplify equally efficiently across a broad range of template dilutions by standard curve (listed in Table S1).
For such samples, the same dCt values were yielded for a broad range of template input, from 150 ng/reaction down to 1.5 ng/reaction (Figure 3A).
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The same strategy can be extended to a broad range of templates.
The same strategy can be extended to a broad range of templates, monomers and surfaces.
The assay allowed for highly accurate quantification of the SHOX2 DNA methylation over a broad range of relative methylation of the template DNA.
Glycyrrhetic acid, a pentacyclic triterpenoid, possesses a broad range of pharmacological activities and serves as template to synthesize many bioactive drugs.
Scaling down these materials to the nanoregime has enabled their use in a broad range of applications including catalysis, spin-crossover, templating, biosensing, biomedical imaging, and anticancer drug delivery [10, 11, 12].
These findings are relevant to a broad range of nanoporous carbon surfaces prepared with hard templates.
Reticulated polymer foams are commercially relevant materials suitable for a broad range of applications including impact absorption, environmental protection, biological scaffolds and sacrificial templates for the fabrication of functional materials.
Unlike existing methods, which define a different HMM for each relationship type, CARROT defines three HMM templates from which it generates HMMs for a broad range of relationships.
These mechanisms may both serve to describe and explain the specific difficulties that military families experience and provide a broader template for understanding the risk processes that underlie families' adjustment to a broad range of traumatic and other stressful circumstances.
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