Thermodynamic analysis of numerous GA and MBP variants suggests that the key to our approach involved stabilizing the modified MBP and OspA subdomains via external interactions with neighboring substructures, indicating that subdomain interactions can stabilize alternative folds over a broad range of sequence variation.
We show its superiority over conventional methods for phylogenetic studies with a broad range of sequence divergence in and beyond the twilight zone of remote homology.
Although exhibiting a broad range of sequence variations, rubber-like elastomeric proteins share common properties in combining repetitive, highly disordered sequence elements with cross-linking motifs.
We used this dataset to search for signals of thermoadaptation using a broad range of sequence-based methods that have been proposed in the thermostability literature.
The polypeptides encoded by the platyhelminth GPx genes showed a broad range of sequence identity either to one another or to the other eukaryotic PHGPx-like proteins (21 87%).
This careful choice of PH domain pairs then justifies this experimental comparison, irrespective of the broad range of sequence divergence within PH domain family known to exist across many species.
Despite the high selectivity of dSfmbt in discriminating between different lysine methylation states, it is able to recognize mono- and dimethylated lysine in a broad range of sequence contexts: dSfmbt also binds histone peptides mono- or dimethylated at H3K4, H3K9, H3K27 or H3K36 with affinities ranging between 1 and 16 μM (Table II).
The library generated by Robine et al. [ 24] from adult mouse testes lysate, containing both pre-pachytene and pachytene piRNAs, is one of the few publicly available libraries of small RNAs that covers a relatively broad range of sequence lengths 15-366 nt).
A broader range of sequences, including mitochondrial and large viral genomes, has been surveyed by Karlin et al [ 3, 4, 6, 8, 9, 12, 17] using 50 kb and larger windows.
