This study aims to (i) test for genetic differentiation between strains of a broad range of origins, (ii) investigate the evolutionary origin of clinical isolates, and (iii) identify an association between a virulence-related trait and pathogenicity.
The population structure of the examined strains have not been well characterized and their selection has been from a very broad range of origins and/or biased by certain characteristics such as specific antibiotic resistance mechanisms.
Mann presented data obtained using the Orbitrap-Elite platform where more than 10,000 proteins were identified in a single lysate from a human HeLa cancer cell line [ 7] and from 10 other human cell lines, representing a broad range of organs of origin.
JCV is a human PyV that has been associated with brain tumors, including a broad range of glial-origin tumors.
The cancer-staging examinations were performed on a broad range of cancer origins; the evaluated diseases are listed in detail in Table 2.
Although there are hexosaminidases which are able to degrade a very broad range of substrates regardless of origin and type (e.g. jack bean hexosaminidase, Li and Li 1970), the enzymes showing high specificity for a defined, single terminal β-GlcNAc residue were not known until the detection of the FDL hexosaminidases in insect cell extracts (Altmann, Schwihla, et al. 1995).
Compositionally, the project covered a broad range of species/populations of different origins (marine, freshwater, migratory and glacial relict species), with different life history traits and environmental preferences in as many subsystems as possible.
The genomic assemblies available for 41 S. cerevisiae strains were sampled: these consisted of the reference strain S288c and a set of 40 additional strains covering a broad range of ecological and geographical origins (Table 1).
The professionals' panel was not large, either, but it was very diverse, including respondents with a broad range of professional backgrounds, training, and country of origin.
