In contrast, a protein that is concentrated in particles should show a broader range of intensities: low outside of the particles, and high in the particles.
Protein-bound microarrays were scanned to detect Alexa Fluor 647-conjugated anti-GST using at least two different laser power settings to capture a broad range of feature intensities and to ensure signal intensities were below saturation for all spots.
The human visual system manages to provide a usable signal over a broad range of light intensities.
There was a broad range of RFP intensities in the Ly6Clo monocytes (Figure 2I), though many were as bright as those in the Ly6Chi population.
If this alternative hypothesis is correct, reducing predator abundance could result in increased survival of non-territorial or peripheral individuals, but the density of coral-killing algal gardeners should remain approximately the same over a broad range of predation intensities.
The baseline transmission model presented here was fully parameterised using datasets from several Sub-Saharan settings, encompassing a broad range of transmission intensities, and its parameters were subject to an extensive sensitivity analysis in [27].
Even assuming the normal biological variability, we wonder as to whether this broad range of effect intensities can be justified by changes in the perfusion system.
There was a broad range of signal intensities with a statistically significant loss of STAG2 expression in the tumor tissue (Wilcoxon rank test) and complete absence of STAG2 staining observed in 15 (4.3%) patients (Additional files 4 and 5).
