The hydrolysis of extracellular ATP to adenosine is orchestrated by ectonucleotidases, especially CD39 and CD73, which are expressed by a broad range of cells, including myeloid, endothelial and regulatory T cells8,9,10.
While many aspects of both homologous recombination (HR) and nonhomologous end joining (NHEJ) are shared by a broad range of cells and organisms, some features are specific to individual situations.
Immunoglobulin-like transcript 1 (isT1/LIR-7/LILRA2/CD85h) is one of the activating receptors in the ILT family whose members have been reported to regulate a broad range of cells involved in the immune response.
Cytokines are loosely categorized as signaling proteins, which are released by a broad range of cells.
A potential downside to this approach is the possibility that the modified VSV will kill cells that aren't infected by HIV. Rose believes that won't happen because he has stripped VSV of its own surface protein, which is what allows it to infect a broad range of cells.
IL-17 acts on a broad range of cells to induce the expression of cytokines (IL-6, IL-8, GM-CSF, G-CSF), chemokines, and metalloproteinases [40] [43].
Our technology is a promising method of cell surface engineering that is applicable to a broad range of cell therapies.
Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types.
Among all currently available viral vectors, adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.
Therefore, generating cell lines stably expressing affinity tagged bait proteins in a broad range of cell types and cell lines is important for identifying PPIs that are dependent on different contexts.
