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Many Tc variants have been produced, both to overcome bacterial resistance and to achieve a broad range of binding strengths.
By the adoption of different experimental strategies (column chromatography, simple partition equilibrium, solid-phase immunoassay and biosensor technology protocols) quantitatiative affinity chromatography can be used to characterize interactions governed by an extremely broad range of binding affinities.
Notably, UBF contains an array of six HMG box domains that exhibit a broad range of binding affinities for DNA [ 69].
They observed a broad range of binding potential and specificity across cell types, implying a high level of variation in cell surface glycans within mammalian cell types.
The protein mutagenesis experiments therefore give a more nuanced view of how EF-Tu achieves its broad range of binding affinities for different tRNA bodies than was previously obtained from tRNA mutagenesis experiments.
The binding of protein onto nanoparticles showed a broad range of binding efficiencies, varying in the protein:nanoparticle ratio between 1 to 1.8, and indicating that the activated nanoparticle had attained protein loading saturation.
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Many enzymes have broad ranges of binding affinity for different substrates that allows them to affect a variety of cellular pathways.
However, ligand-based approaches often cover a broader range of binding conformations, but only a small part of possible binding site space at the protein, which is a reason to prefer structure based methods.
Low complexity domains, on the other hand, provide a means for more dynamic interactions with a potentially broader range of binding partners (Fig. 2).
Here we describe a computer model, TEPITOPE, that enables the systematic prediction of promiscuous peptide ligands for a broad range of HLA binding specificity.
Herein, we report a class of slightly larger peptides (up to 34 amino acids long) that contain widely disparate peptide sequences, resulting in a broad range of cellular binding and radioisotope uptake properties.
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