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Both enzymes are active and stabile over a broad pH range and also their activity is completely inhibited by PMSF suggesting that they both are serine proteases.
The ammonia treatment caused an increase in positive surface charge density over a broad pH range; and this was reflected in a higher pHpzc and pHIEP.
Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation.
Subsequent characterization revealed YDR051c/Det1 to be an acid phosphatase with broad substrate specificity, whereas YOR283w has a broad pH range and hydrolyzes hydrophilic phosphorylated substrates.
Moreover, TEVp has high catalytic activity [6], works over a broad pH range, and retains activity at temperatures from 4 37°C.
To address such question, we carried out far-UV CD and fluorescence thermal-denaturations over a broad pH range and, in addition, DSC measurements at pH 7.3.
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Besides, the lipase-NPG biocomposite exhibited high catalytic activity in a broader pH range and higher temperature than that of free lipase.
The mutants retained more activities over broader pH ranges and higher temperatures and catalyzed d-glucose at higher efficiency (5800‒12,667 M−1 s−1 for the mutants versus 5083 M−1 s−1 for the wild-type).
Paenibacillus Q8 has polymer-degrading enzymes that confer it cellulolytic, hemicellulolytic and amylolytic activities under a broad pH range (4 10) and under high As III) and As V) concentrations (up to 750 and 1,000 mg/L, respectively) (Delavat et al. 2012b).
Thermoanaerobacterium saccharolyticum JW/SL-YS485 is a highly xylanolytic anaerobic thermophile that grows within a broad pH range (3.85 6.35) and over a broad temperature range (30 66°C), and can use xylan as the sole carbon and energy source.
Major activities of ~400, 120, and 32 kDa in TES were relatively similar over a broad pH range (5.5 9.0) and all these were of the serine-type as leupeptin abolished gelatinolysis.
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