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By regulating the hydrolysis time (10 180 min), a broad molecular weight range of SF was obtained (263.1 82.7 kDa) Gel point increased with a decrease of SF molecular weight.
Therefore, a broad molecular weight range as prepared here by transition metal initiators is hardly accessible by the amine-initiated NCA polymerization.
SEC was performed at room temperature using five columns (PLgel 10 μm 10, 10, 10, and 106 Å, plus a larger 107 Å Shodex column) to cover the broad molecular weight range of the carbanilated celluloses.
However, our results are not unexpected given the large range of protein complex sizes that exist within the membranes of mitochondria and are consistent with the broad molecular weight range of extracted complexes detected by BN-PAGE.
This de-glycosylation has been shown to alter the Western blot appearance of FD from a broad molecular weight range of 40 44 kDa to a single 26 kDa band, thereby allowing pro-FD and factor FD to be distinguished by size [ 13].
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The broad molecular weight size range measured was likely from the presence of 12-, 18-, 24- and 36-mers generally described – and isolated – as the HMW complexes.
GPC analysis showed that PDMAEMA homopolymer and the above mentioned two block copolymers possessed narrow molecular weight distribution (¯¯¯¯Mw/¯¯¯¯Mn) in the range of 1.15 1.34, while PMMA homopolymer had a little broad molecular weight distribution of 1.29 1.60.
This extended the molecular weight range of chromatography to polypeptides, proteins, and high-molecular-weight polymers.
Extremely high molecular weight polyethylene could be produced (approaching 1 million Da), with industrially desirable, broad molecular weight distributions.
The resultant copolymer with 1 gave relatively broad molecular weight distributions, consisting of high and low molecular weight copolymers.
This allows structure searches within a specified molecular weight range.
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