Within the broad distribution of cells expressing TG6, two clusters of cells of different size were apparent and were gated (Supplemental Fig. 4, R1 and R2).
In both groups, a similar and broad distribution of single-cell IFNB1 gene responses was observed (Supplementary Figure S3).
Because cells dependent on EBV-derived plasmids have a broad distribution of plasmids per cell; more cells can lose some plasmids per generation than succumb to the selective disadvantage.
High internalization efficiencies are achieved (up to 500 molecules per cell; Crawford et al. 2013), although there is a broad distribution of internalized molecules per cell.
It may also explain why the tobacco conversion enhancement due to E1 expression was not apparent at the highest pretreatment severity; that is, the innermost layers of the tobacco cell wall were not as disrupted by pretreatment as were those of stover, which had more broad distribution of E1 throughout the cell wall.
The distributions in all four subcloned populations were similarly broad, with the average number of plasmids centered on 6.7 9.6 plasmids in aphidicolin-treated cells, and 11.9 15.1 plasmids in nocodazole-treated cells, indicating that the broad distribution of viral genomes in 721 cells was generated within only 25 generations.
The cellular stochasticity of gene induction, which we investigate, is not limited to the specific interferon-induced gene we have studied; a broad distribution of copy numbers across cells is to be expected for other interferon-stimulated genes.
The DU-145 showed a broad distribution of the protein within the cells, with a more intense localisation in the nuclear region.
Heat maps of curvature allow us to directly visualize the broad distribution of nuclear blebbing in a large cell population.
These results show that the wide variety in the relative mobility of telomeric restriction fragments in the gel is due to a broad distribution of telomere lengths from the population of cells.
