In litchi, we identified a total of 197 gene targets for 167 known miRNAs using degradome analysis (Table 2, Additional file 5), and the majority of these targets are conserved in plant species, indicating broad conservation of the known miRNA regulatory roles in plants.
These miRNAs might represent two classes of conserved miRNAs, i.e., miRNAs in broad conservation families and poor conservation families.
TargetScan, which uses site and miRNA conservation across different species as selection criteria, predicted 43 individual miRNAs with broad conservation among vertebrates, targeting a total of 14 different conserved miRNA binding sites.
As with MbnB, multiple alignment of MbnC homologues confirms the broad conservation of several regions of the gene, but the relationship between the conserved regions and MbnC's potential role in biosynthesis of thioamide and nitrogen-containing heterocycles remains unclear.
In Sacramento, Mr. Davis met with Gov. John Kitzhaber of Oregon and Gov. Gary Locke of Washington, and they issued a statement pledging a cooperative effort to solve the crisis, in large part through broad conservation programs.
To woo young men away from the Taliban, he wants to create a broad "conservation corps" to employ 300,000 men — approximately one from every family — to build roads and bridges in the impoverished tribal region.
The Forest Service and the Interior Department would provide overall supervision, making sure that the thinning strategy is not used to expand commercial logging beyond federally mandated levels or undercut broad conservation goals.
Both native and alien species must be considered when formulating broad conservation aims in rangelands (Milton et al. 2003).
The achievement of these broad conservation objectives will likely require large areas, and stretch limited funds for MPA implementation.
In a recent study, Kelly et al. showed how indicators can provide a link between broad conservation targets and local-scale implementation (11).
We encourage those responsible for forest management to re-think prescriptive placement of fixed-width forested buffers around all lakes, and instead consider a landscape-scale planning approach that determines the appropriate placement of uncut forest on the landscape to meet broad conservation objectives.
