These observations suggests that the primary, high level mechanisms of 14-3-3 14-3-3 14-3-3ificity are a broad binding site that allows multiple trajectoriespecificityefore interareion with different residues) and side chain rearrangement to accommodate different peptide sequences.
Finally, clusters that exhibited a strong peak of gene expression (log2 > |3|) at 6 h APF (or a dip in the case of cluster 28) were typically associated with Broad binding-site enrichment.
We also compared TFBS of sex-biased genes: in healthy kidneys there was a common enrichment for Broad-complex_3 binding sites, while in diseased kidneys, there was enrichment for transcription factors Broad-complex_1.
TFs bind to a broad spectrum of binding sites with different affinity and change targets widely among species.
The uniquely mapped HLP1-CLIP tags showed a broad range of binding sites to sense targets (1 934 562), encompassing 5′-UTR (2.69%), coding sequences (CDS, 18.61%), introns (23.18%), 3′-UTR (26.11%), intergenic regions (27.03%), pseudogene (0.80%) and noncoding gene exons (1.58%; Figure 2B).
In fact, it is conceivable that the brain may be a common site of iRBC adhesion and sequestration because, constituting the blood brain barrier, the cerebral endothelium is extraordinarily rich in receptor and transporter molecules offering a broad variety of binding sites for iRBCs, which all in all are armed with an even broader variety of adhesion ligands (Miller et al., 2002).
This is not to say that transcription factors involved in one cluster didn't play any role in the response in other clusters (e.g. note the broad presence of TF binding sites for NF-κB, HNF-1, or PolyA), but those associations didn't reach statistical significance in our analysis.
These sonicated fragments are often much longer than the actual protein-binding site and, thus, the sequence tags of the ChIP-DNA distribute in broad regions around the actual binding sites.
Fittingly, over a dozen folds and a broad spectrum of binding site architecture, ranging from shallow grooves to deep pockets, have developed sugar-binding capacity.
Akirin1 has between one and four putative 14-3-3 14-3-3 14-3-3 across a bindinghylogenetic range of vertebratesitesneracrossn regions conserved with at least one Akirin2 protein.
