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For E. coli TY2482, AAMG, RAST and BG7 [ 10] annotations were compared against the BROAD annotation.
Briefly, nTARs were identified by comparing the current broad annotation with the annotation obtained from the cufflinks pipeline (filtering class code u) using cuffcompare (option -r).
We also provide information which clone was used to prepare the FISH probe; the classification into broad annotation classes ('no signal'ubiquitousous'specific'ic', see 'Results'.
While all KEGG pathways were considered for enrichment, to avoid broad annotation terms, only GO-BP categories with fewer than 1,500 genes (mouse annotations) were retained [ 63].
These included BC1G09040_09041.1, BC1G07441_07442.1, and FGSG11659.3 and FGSG11630.3 which we conclude represents a single gene corresponding to the MIPS and version 2 broad annotation (FG_00042.1), (Additional file 2).
It unifies broad annotation and flexible filtering strategies in a user-friendly online interface and at the same time provides direct integration with the semi-automatic analysis capabilities of platforms such as Galaxy.
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Thus, the broad manual annotation of the MeSH descriptors can enhance detailed automated event extraction.
Additional file 1: Data Set S1 is rank-ordered by p-values, consequently the top terms represent broader general annotations with less information.
Automatic annotations are made using algorithms that consider gene product properties, such as orthology, domains and sequence similarity [ 5, 9], and they provide a broad coverage of annotation and cover a significantly larger taxonomic range than manual annotations.
Additionally, MToolBox provides the user with essential analyses of reconstructed mitochondrial genomes, i.e. haplogroup assignment and variant prioritization, exploiting a broad collection of annotation resources.
Using these three strategies, 842 genes with possibly incorrect gene models or alternative splicing were identified when compared with the Broad F. graminearum annotation.
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