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Xgal staining is useful for a broad analysis of expression patterns.
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To provide a broad analysis of gene expression changes in developing embryos from a solanaceous species, we produced amplicon-derived microarrays with 7741 ESTs isolated from Solanum chacoense ovules bearing embryos from all developmental stages.
These studies provide a broad analysis of differential Burkholderia sRNA expression profiles and illustrate the complexity of bacterial gene regulation in response to different environmental stress conditions.
Subtraction hybridization (Hedrick et al., 1984) and the generation of tissue-specific cDNA libraries were the initial strategies used to pursue a broader analysis of gene expression and promote the discovery of novel tissue-restricted genes.
We undertook a broader analysis of rapamycin effect on gene expression so as to identify genes, groups of genes (ontology analysis) or pathways that could account for rapamycin responsiveness.
Such transcriptome-based data sets will help to facilitate ongoing and new studies ranging from analysis of expression patterns of individual genes to broader comparisons of genome-wide gene expression patterns among N. vitripennis and other organisms.
Through phylogenetic analysis of expression network modules, we found a correlation between stronger co-expression and broader conservation of genes.
Our analysis of expression profiles of human genes bearing intronic snoRNAs indicates that snoRNA-containing genes have a broad expression profile.
The broad-spectrum analysis of the gene expression performed here indicated that, similar to the genes coding for transporters, the molecules involved in iron and zinc transport also appear to exhibit different modulations and roles in the cell (Table 2).
GLUT1 protein is expressed in most melanoma metastases with a broad range of expression levels as shown by Western immunoblot analysis [33].
Despite the growth inhibitory effects of HDAC-i demonstrated in various cell lines including bladder cancer cells, a broad expression analysis of this attractive target has not been conducted yet [ 12- 16, 31, 32].
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