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This was adjusted for 15% non-response rate; bringing the total sample size to 440.
An additional 125 individuals from BS31 were analyzed bringing the total sample count to 575.
It was further adjusted for a 10% non-response rate; bringing the total sample size to 420 respondents.
To protect against subject attrition, we will recruit an additional 6 families (i.e., ≈20% of the total sample size), bringing the total sample size to 40.
Field sampling on the additional four cities was completed on 18 June 1994 (Maiolo 1994), bringing the total sample size to 1,564 people (507 males, 1,057 females).
Of the 163 individuals registered (95 residents, 68 supervisors), 13 refused and 32 did not react to the request of using their e-mail address, bringing the total sample at 118 YHC respondents.
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We used an incubation buffer (10 mM Tris and 80 mM glycine adjusted with acetic acid to pH 7.8) to bring the total sample volume to 20 μL.
Although the amount of fungi varied per swab sample, G. destructans was recovered from all four swabs, henceforth considered as a single sample, bringing the total of samples analysed to 23.
One male participant from the home arm died after the enrolment interview and was replaced bringing the total enrolment sample to 41.
Duplicate samples of three herbarium specimens were included from the DNA extraction stage onwards, bringing the total number of samples to 64.
Loci that showed evidence for association were followed up in the additional 14 568 cases and 15 274 controls, bringing the total number of samples analyzed to ∼48 000.
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