Sentence examples for bringing the final volume from inspiring English sources
Unreacted IA was then neutralized by the addition of 10 mM DTT in 100 mM AmBic and the urea content diluted to 4 M by bringing the final volume to 500 μL by the addition of 100 mM AmBic.
The following day urea content was further diluted to 1.4 M by bringing the final volume to 1.43 mL by the addition of 100 mM AmBic and trypsin was then added at 1 50 (trypsin:sample, w/w) and the digestions incubated for four h with shaking at 37 °C.
For PSD preparation, a 500 µl aliquot of SPM was diluted with ice cold 0.1 mM CaCl2 to 2.5 ml, followed by the addition of 2.5 ml of 40 mM Tris-HCl pH 6 supplemented with 2% Triton-X 100 and protease and phosphatase inhibitors, bringing the final volume to 5 ml with 20 mM Tris and 1% Triton-X 100.
The samples were cooled before the addition of 1.5 ml of 100 mmol/l citrate buffer pH 4.8, bringing the final volume to 3 ml (~2% solids (w/v)).
Then, 5.0 ng purified PP2A (Upstate Biotechnology, Lake Placid, NY, USA) were added to the reaction mixture, bringing the final volume to 100 μl and incubated at room temperature.
The tube with the particles was then placed in a bath sonicator (Fisher Scientific FS30) for 5 min, and afterward, 25 μL of aqueous ammonium hydroxide (28 30%) was added to the reaction mixture, bringing the final volume to 1.5 mL.
Reaction was finished by cooling down the tubes on ice, and finally 20 μl of water were added to bring the final volume up to 40 μl per tube.
The reaction system included 150 ng of sample total RNA and 1 μl of the master mix plus RNase-free water to bring the final volume to 5 μl.
A 0.5 mL aliquot of iodine reagent (0.1% I2 + 1% KI) was added to the samples and then distilled water was added to bring the final volume to 50 mL.
Assay buffer was used to bring the final volume to 10 ml and the worm suspension was allowed to settle for 5 minutes.
