Two participants with BD-I were excluded following uncertain euthymic status at scanning, bringing the final sample to 20 HC, 16 BD-I, and 19 BD-II.
Only participants scheduled for a morning blood draw had triglyceride and plasma glucose data because these samples were obtained after an overnight fast, bringing the final sample size to 1,367 (709 men and 658 women).
We excluded study participants with problematic heart rate measurements from the analysis (as described below, n = 56 for BC and n = 65 for PM2.5), which brought the final sample size to 540 for BC and 475 for PM2.5.
However, 3 cases were confirmed dead and 3 others were conveniently sampled for pre-testing the questionnaire bringing the final sampling frame to 99 confirmed cases.
For each sample, 35 100 μg total RNA was diluted 2 1 in digestion buffer (50 mM Tris pH 7.5, 5 mM MgCl2, 0.5% Triton x-100, 1 mM DTT, 20 U/ml SuperaseIn, 20 μg/ml emetine, 15 mM CaCl2, and 3 U micrococcal nuclease [Roche Applied Science, Indianapolis, Indiana] per μg of total RNA in the sample), to bring the final concentration of NaCl to 100 mM and CaCl2 to 5 mM.
cDNA (2 μl) was added to bring the final volume of the real-time PCR sample to 25 μl.
A larger amount of extract was taken for evaporation to compensate for the reduced amount of sample processed and to bring the final extract concentration to 0.2 g mL−1.
A 0.5 mL aliquot of iodine reagent (0.1% I2 + 1% KI) was added to the samples and then distilled water was added to bring the final volume to 50 mL.
Super mix (18 µl) was added to bring the final volume to 20 µl.
Methanol was then added to bring the final ratio of water to methanol to 85:15.
