Sentence examples for bring the final concentration from inspiring English sources
To bring the final concentration to a single-strength BPW, 10 mL of 10-times concentrated BPW was added to the rinsate.
A mix of water and 5% aqueous methanol with 0.1% formic acid was added to bring the final concentration of methanol to less than 1% (total volume 4 mL).
Sonicated chromatin was diluted in lysis buffer without SDS to bring the final concentration of SDS to 0.05%.
For isonicotinamide (INAM) cultures, a 1 M INAM stock was used to bring the final concentration to 25 mM INAM in the SC media.
EDTA was added to bring the final concentration to 10 mM, and the probe was heat denatured at 95°C for 5 min. Arrays were hybridized with probes in ULTRArray hybridization buffer (Ambion, Inc., Austin, TX) overnight at 55°C.
Long PCR for amplification of 3-8 KB fragments were performed the same way except that the 10X buffer was supplemented with additional MgCl2 to bring the final concentration to 25 mM and the extension time was increased to 6 min.
After 30 min, 60 μL of osmolytes or 60 μL of ddH2O was added to bring the final concentrations of 3.4 μM BlTreA, 50.4 mM GdnHCl, and 0.30 1.25 M sugars.
After this first incubation period, 10 μl of MTT labeling reagent was added to each well, bringing the final concentration of MTT labeling reagent to 10 mg/ml.
A 60 μL aliquot was added to each well, which contained 300 μL of cell lysis buffer, bringing the final concentration of substrate to 100 μM in each well.
Prior to digestion, proteins were reduced in 5 mM dithiothreitol for 30 min at 55 °C and subsequently alkylated in 10 mM iodoacetamide at room temperature in the dark for 30 min. Alkylation was quenched with a second aliquot of dithiothreitol, thus bringing the final concentration of dithiothreitol to ∼10 mM.
40 μl of stock N2 was added per 4 ml of growth medium, bringing the final concentrations to 20 nM of progesterone, 100 μM of putrescine, 30 nM of sodium selinite, 0.1 mg/ml of BSA and 100 μg/ml of transferrin.
