Cross-referencing the data (unscientifically) reveals the statistically most dangerous flights to take: by month by airline by plane type by country by seat" David has a collection of brilliant data visualizations now available on Flickr.
After protein visualization with Coomassie Brilliant Blue, the gels were dried and subjected to autoradiography.
The gel was stained with Coomassie brilliant blue R-250 for visualization.
The resulting MtrC sample (87 × 10−6 m MtrC in 20 × 10−3 m HEPES, pH 7.5) was subject to SDS-PAGE followed by protein visualization using Coomassie Brilliant Blue staining.
Total soluble protein extracted from leaf or seed was diluted with 4× loading buffer (0.2 M Tris-HCl, pH 6.8; 0.8 g SDS; 40% Glycerol; 5%β-mercaptoethanol; 50 mM EDTA; 8 mg Bromophenol Blue), boiled for 5 minutes and separated on 15% SDS-PAGE with 10-50 μg protein/lane followed by Coomassie Brilliant Blue Staining for protein visualization.
Gels were stained with Coomassie Brilliant Blue R 250 (CBB) for visualization of the protein bands.
After separation the proteins were either stained with Coomassie Brilliant Blue staining (for total protein visualization) or transferred to a PVDF membrane (ImmobilonP, Millipore) by the semi-dry transfer method (Trans-Blot SD Semi-Dry Transfer Cell, Bio-Rad) for Western Blot analysis.
Visualization was achieved either using Coomassie Brilliant Blue staining or Pierce® Silver Stain Kit (Life Technologies, Darmstadt, Germany).
To verify the production of rEhMCF and its purity, 1/100 volumes from each fraction were analyzed by SDS-PAGE, followed by visualization through either autoradiography or Coomassie brilliant blue staining.
Visualization was conducted by staining with Coomassie Brilliant blue (Laemmli, 1970).
