"Claudius," a 1986 abstract painting by Mr. Richter in brilliant yellows and greens, was estimated at $1 million to $1.5 million.
Because the S100A9 showed weak silver-staining, its purity was estimated by Coomasie Brilliant Blue staining and densitometric analysis to be greater than 95%.
The protein concentration was estimated by the Coomassie brilliant blue (G-250) dye-binding method [ 21] using Bio-Rad dye stock solution with bovine serum albumin (Sigma) as a standard.
Most fractions were more than 98% pure as estimated by SDS-PAGE Coomassie Brilliant Blue staining.
The theme fit well with Kughn's collecting interests: He has molded his 80-car portfolio, worth an estimated $8 million, into a showcase of brilliant American engineering.
The GST-PCT+N-GFP complex was purified to >95% homogeneity, as estimated by SDS-PAGE and Coomassie brilliant blue staining (Fig. 1A).
In a brilliant piece of deduction, Pat Phelan and Florian Seroussi of telco startup Maxroam estimate on their blog that time spent on Twitter will cost $13.5bn in lost productivity 2008.
Bands corresponding to the activity were visualized by negative staining using Coomassie Brilliant blue R-250 (Bio-Rad Laboratories, Richmond, CA, USA) and molecular weights were estimated by reference to prestained SDS-PAGE markers.
The separated proteins were visualized by staining with Coomassie Brilliant Blue R-250 (Sigma, USA) and apparent molecular weights of xylanases were estimated by comparison with the standard proteins using the Quantity One software.
Henry Wyndham, chairman of Sotheby's Europe and a brilliant auctioneer, opened the bidding at £22.5 million, a mere 10 percent below the low estimate.
Brilliant, brilliant, brilliant.
