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The reason is that the red autocorrelation curve and thus the brightness determination are affected by cross-talk.
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The light power was adjusted to avoid reflection and obtain images with adequate brightness for determination of the plaque color.
"But I was impressed by the brightness, the alertness and the determination, and felt he would be ready to do serious work".
"But I was impressed by the brightness, the alertness and the determination, and felt he would be ready to do serious work". And Mr. Dorfman recalls the encouragement Ms. Myers gave him.
Our preferred method of quantification employs the determination of a brightness area product over the brain region of interest (8).
11 In our study, we used a well-recognized objective tool, the Chroma-Meter, to assess skin color (not only skin pigmentation) by a determination of its brightness (L*), saturation (chroma C*), and hue angle (basic color) values.
Determination of L* (brightness), a* and b* (green red and blue yellow chromaticity coordinates), individual typology angle, and C* (chroma) and h* (hue angle) parameters using a Chroma-Meter demonstrated that THBG is able to modify skin color while quantification of ultraviolet (UV) spots by VISIA-CR™ confirmed its photoprotective effect.
Hendrickson's determination to see a glimmer of brightness is especially obvious when he encounters 83-year-old John Ed Cothran, one of the sheriffs and a contemporary of H. C. Strider, who helped pull Till's mutilated body from the Tallahatchie River.
It is difficult to determine whether the observed range of brightness ratios represents true cell-to-cell variation in the proportions of different oligomeric states or errors in the determination of cluster densities and fraction FRET values.
As a result, determination of the surface slope from the apparent brightness of a given pixel can be confounded by uncertainly in such variations.
For example, low cross-excitation and high acceptor brightness (i.e., high quantum yield and high absorption) are important for SE determinations, whereas for FLIM detection these factors are less important.
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