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Of these potential strains, 4 strains showed the brightest fluorescence (Table 1).
During imaging, the exposure time was adjusted to obtain images in which the brightest fluorescence spots have pixel values larger than half the full pixel ADC range.
The brightest fluorescence in the lamina propria, which was attributed to eosinophils (beta approximately 10 -2.5 10 -2.5evinus studies, was also observed in other granular structures (beta uprevious-1.4)).
Images with meaningful fluorescence were found beginning at Hour 36, and ascended to the peak plat period with the brightest fluorescence between Hour 54 and Hour 72.
Both hypotheses are compatible with the fact the zone of the brightest fluorescence in lancelets corresponds to the anterior part of the adult animal that is exposed during feeding.
Similar(55)
We characterized variants of the red fluorescent protein eqFP611 with bright fluorescence emission shifted up to 639 nm.
Each line first sampled the non-fluorescent background of the pleural arch, then the bright fluorescence, and finally back into the surrounding non-fluorescent region.
Syto-62 is a fluorescent nucleic acid stain that exhibits bright fluorescence upon binding to nucleic acids and is retained in viable cells.
We confirmed that this Ca2+ red-fluorescent probe showed high cell-membrane permeability with bright fluorescence.
pcDNA-GW/EmGFP-miR encodes a red-shifted variant of wt green fluorescent protein (GFP) that has been optimized for brighter fluorescence and greater expression in mammalian cells.
This molecule has recently been used to realize fluorescent self-assembled monolayers with pH sensitive multicolor and bright fluorescence [23].
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