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LG Optimus bright L-07C (world's brightest cell phone display (4 inches, 700cd/m2), 9.5 mm thin, Android 2.3, Japan version of the Optimus Black- more here).
Separately, we took PC3M-luc-C6 which was one of the brightest cell lines (∼250 photons/sec/cell) generated by us thus far (using multiple rounds of transfections by pGL3) and compared it with 4T1-luc2-1A4 cells, by implanting both into SCID-bg mice (Figure S3).
The 5%% brightest cell population was isolated by a modified FACSAria II flow cytometer (BD BioSciences).
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The brightest cells are typically those just entering mitosis; the daughter cells subsequently halve their relative fluorescence from that seen in G2 as the chromosomes are segregated.
This raises the possibility that arteriolar Nes-GFP+ cells (Kunisaki et al., 2013) might not completely coincide with the brightest cells isolated by FACS.
In fact, when administered within 5 min of each other, the hypoxic fraction of the Hoechst brightest cells increased to almost 70%.
Pooled values from 1 to 4 h were significantly elevated in the brightest cells compared to the dimmest population when analyzed using a paired t-test.
The degree of purity of the sorted brightest cells was confirmed by FACS and by quantitative Real Time PCR (qRT-PCR) analyses of CM- and CF-specific markers.
In each of the micrographs, we counted the neuronal number, with the criterion of neuron being one having a phase-bright cell soma.
Healthy cells were identified as having smooth, phase-bright cell bodies and intact neurites.
The endothelium was revealed with OCM in Fig. 2E by high contrast between bright cell bodies and dark cell membranes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com