Sentence examples for brightest band from inspiring English sources

If amplification was successful in only one of the two species, the pair generating the brightest band for that species was selected.

The largest and brightest band was chosen to clone into the pGEM-T Easy vector for sequencing.

We therefore extracted the desired PCR product, (identified as the brightest band nearest to the target size), from an agarose gel using Qiagen's QIAquick Gel Extraction Kit according to the manufacturer's protocol.

The molecular ladder (M) of the gels was separated by 100 bp bands with the first band at the bottom being 100 bp; the brightest band near the top of the ladder is at 600 bp.

Amplification conditions included an initial step at 95°C (3 min), followed by 25 repetitions of the following cycle: 95°C (30 sec) gradient temperatures between 56° to 62°C (30 sec), 72°C (20 sec), then concluded by a step at 72°C for 5 min. Amplicons were migrated and visualized on 2% agarose gels in order to confirm optimal melting temperature as determined by the brightest band.

To test the amplification quality, six of the best PCR products (corresponding to the brightest bands in a 1% agarose gel) were selected for sequencing: Linum, Anethum, and Senna generated with an annealing temperature of 58°C, with and without Enhancer.

Burns asked to rewatch the shot that follows Miller: the bridge, as seen from the Brooklyn side, against an evening sky that's dark and threatening, except for a bright band above the horizon.

According to the theory, a bright band is produced when the crests (and troughs) of the waves from the two slits arrive together at the screen; a dark band is produced when the crest of one wave arrives at the same time as the trough of the other, and the effects of the two light beams cancel.

The ridge information manifests as a bright band.

This area is recognized as the bright band in Figure 1D.

Cloud systems with bright band features consist of the higher reflectivity values and cloud liquid water near 5 km altitude and reflectivity values show a sharp decrease just below the bright band.