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Alignment of images using Spc42-mCherry present in the strain showed that the brighter focus (focus 2) was located at a distal region of the bridge interior to the N-terminus of Sfi1.
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The GFP-tagged U1-70K alshowedwed a similar fluorescence pattern in the Arabidopsis mesophyll protoplasts with brighter foci and a substantial nucleoplasmic distribution (Figure 1).
We observed an increased number of γH2AX foci, and brighter foci, in 898- or 233-treated cells compared to the untreated control cells.
Interestingly, while the positions of TssL spots remained relatively fixed, especially for brighter foci, spot intensities fluctuated over the 100-ms range, especially in weaker spots.
In addition, the Rad51 and DNA-PKcs foci-positive cells transfected with control shRNA contained both more and brighter foci than the cells transfected with NFBD1-shRNA, especially at early time points.
In general, brighter foci detected by FISH are indicative of a higher number of repeats because a greater number of probe molecules hybridize to a target, thereby yielding a strong and clearly distinguishable signal.
Like Poppy, the bright focus of this expansive, moving film, Mr. Leigh isn't one to go it alone.
As would be expected, signal processing emphasises the bright focus and can help to make the comet tail more conspicuous to the operator (Fig. 6).
Matured in oak for 8 months, this wine brings the opulent richness of Semillon into bright focus with the addition of Assyrtiko.
The existence of such schemes came into bright focus when details emerged about the hunt for Osama bin Laden.
Multiple bright foci were rarely observed in a single cell; most positive cells had a single bright focus.
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